东海原甲藻的分子鉴定   总被引：4，自引：2，他引：4  

罗立明  胡鸿钧  李夜光  齐雨藻  吕颂辉  耿亚红  邓光 《海洋学报》2006,28(1):127-131

采用nrDNAITS序列及18S序列两种分子标记,对东海原甲藻和美国国家海洋藻种保藏中心(CCMP)的具齿原甲藻进行分子鉴定,结果表明,两者之间序列非常相似,两者的nrDNAITS序列碱基差异值仅为0.002,nrDNA18S序列的碱基差异值为0.000,根据分子数据,东海原甲藻和美国国家海洋藻种保藏中心的具齿原甲藻应为同一个种.从基因库获取原甲藻的另外3个种nrD-NAITS序列和8个种的18S序列,用计算机软件进行分析和构建分子系统树,结果显示18S序列用于原甲藻的分子鉴定过于保守,而nrDNAITS更适合于该种属界定的分子标准.  相似文献   

利用消去刚度法进行剪切型系统物理参数识别     

王树青  李华军 《中国海洋大学学报(自然科学版)》2004,34(6):1085-1089

提出了 1种能够比较准确地识别剪切型体系的结构参数 (各层质量和层间刚度 )的方法———消去刚度法。该方法从剪切型结构体系的特征值问题出发 ,利用剪切型结构系统质量矩阵和刚度矩阵的特殊形式 ,通过消去各层的刚度系数 ,得到了结构系统的各层质量比表达式。在结构总质量已知的情况下 ,仅利用系统的任意两阶模态信息就可以确定出各层质量 ,并进而计算出层间刚度 ,实现了结构物理参数的递推识别。数值模拟结果表明 ,该方法识别精度较高 ,便于工程应用 ,可以用于类似结构体系的物理参数的初步估计。  相似文献   

Identification of Epinephelus malabaricus and Epinephelus coioides using DNA markers     

WANG Shifeng  DU Jiaying  WANG Jun  DING Shaoxiong 《海洋学报(英文版)》2007,26(1):122-129

1 IntroductionBothEpinephelus malabaricusandE. coioidesbelong to the genusEpinephelus(Perciformes: Ser-ranidae: Epinephelinae).They are warm water reeffishes that have similar distribution ranges as well assimilar morphological characteristics, which have…  相似文献   

Gradation affects basic mechanical characteristics of Chinese calcareous sand as airport subgrade of reefs     

Shen Yang  Xue Shen  Hanlong Liu  Huayang Ge  Xiaoxi Rui 《Marine Georesources & Geotechnology》2020,38(6):706-715

Abstract

Construction of the reefs in the South China Sea is a significant foundation to the secure stability and economic development of China. The construction of an airport runway is necessary for this realization. The calcareous sand is the main primary material in the runway construction. A certain type of calcareous sand near a certain reef of the South China Sea was studied in this paper. To investigate this specific calcareous sand, quartz sand was used as a reference for comparison. Microscopic 3-D imaging, compression and triaxial tests were conducted to test the micro, squeezing and shear properties. The effect mechanism of gradation on the calcareous sand’s compressibility and shear characteristics are discussed from a mesoscopic viewpoint using 3-D morphology. Calcareous sand particles are multiangular and flatter in comparison with quartz sand. The larger the particle sizes are, the more different the two sands’ morphologies are. The compressibility of calcareous sand is greater, and the effect of the coarse fraction (5–1?mm) content in the gradation plays the most significant role in this feature. When the coarse particles’ content is less than 25% and the mass ratio of the middle and fine particles (M) is constant, there is the worst coarse fraction content causing the calcareous sand to be most likely compressed. The worst coarse fraction content decreases with the increase in M, and an empirical formula is proposed. When the gradation, relative density and confining pressure are the same, the peak shear stress and strain of calcareous sand are all at a high level. The effect of confining pressure is manifested in calcareous sand. The shear strength and dilation of calcareous sand are also most affected by the medium coarse fraction (5–0.25?mm) content.  相似文献   

Parameter identification of a compliant nonlinear SDOF system in random ocean waves by reverse MISO method     

R. Panneer Selvam  S. K. Bhattacharyya   《Ocean Engineering》2001,28(9):33

The determination of the drag and inertia coefficients, which enter into the wave force model given by Morison's equation, is particularly uncertain and difficult when a linear spectral model is used for ocean waves, and the structure is compliant and has nonlinear dynamic response. In this paper, a nonlinear System Identification method, called Reverse Multiple Inputs–Single Output (R–MISO) is applied to identify the hydrodynamic coefficients as well as the nonlinear stiffness parameter for a compliant single-degree-of-freedom system. Four different types of problems have been identified for use in various situations and the R–MISO has been applied to all of them. One of the problems requires iterative solution strategy to identify the parameters. The method has been found to be efficient in predicting the parameters with reasonable accuracy and has the potential for use in the laboratory experiments on compliant nonlinear offshore systems.  相似文献   

颗粒测量技术在悬沙测量中的应用与进展     

于连生 《海洋技术学报》2006,25(4):11-14

文中简述了当今悬沙测试技术的进展,提出了六个悬沙测试领域需要统一认识的基本问题,对悬沙测试技术的近期发展趋势作了简短的展望。  相似文献   

Accuracy and precision of fish-count data from a "dual-frequency identification sonar" (DIDSON) imaging system     

Holmes  John A.; Cronkite  George M.W.; Enzenhofer  Hermann J.; Mulligan  Timothy J. 《ICES Journal of Marine Science》2006,63(3):543-555

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结构损伤初步定位的简便方法     

孟海平  杨永春  杨风艳 《中国海洋大学学报(自然科学版)》2006,36(5):785-789

提出将基于动态特性的检测方法与局部物理检测手段相结合的探伤思路。利用动力学方法进行实时监测及损伤区域的粗略定位,再由物理探测方法实现损伤程度和位置的精确判断,从而降低对动态检测方法的精度要求。此外,文中尝试直接由结构的动力响应信号构建能量指标识别结构损伤的方法,不需要进行模态参数识别,算法简单,有望应用于海洋平台、高层建筑等可简化为串联多自由度体系的结构的实时监测和损伤初步定位。  相似文献   

产杀虫活性物质海洋放线菌的筛选和初步鉴定   总被引：8，自引：0，他引：8  

潘云娣  杨文鸽  候温甫  裘迪红 《海洋通报》2006,25(4):92-96

从浙江宁波海域的滩涂泥样中分离到54株海洋放线菌,用卤虫液体测定法进行了杀虫活性菌株的筛选,结果显示多株放线菌具有较高的杀虫活性,菌株XS902,XS904,BL803,BL807对卤虫的ID50高达100以上。同时研究了XS904活性菌株的形态培养特征和生理生化特征,初步鉴定该菌株属于链霉菌属。  相似文献   

Stock identification of Mediterranean horse mackerel (Trachurus mediterraneus) using morphometric and meristic characters   总被引：1，自引：0，他引：1  

Turan  Cemal 《ICES Journal of Marine Science》2004,61(5):774-781

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